Combined Antilisterial Effects of Barberry Extract, Cinnamaldehyde and Nisin

Valizadeh, Soghra; Aliakbarlu, Javad

doi:10.29252/ArchHygSci.8.2.128

Volume 8, Issue 2 (Spring 2019 2019) Arch Hyg Sci 2019, 8(2): 128-135 | Back to browse issues page

‎ 10.29252/ArchHygSci.8.2.128

‎ 20.1001.1.22519203.2019.8.2.2.4

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Valizadeh S, Aliakbarlu J. Combined Antilisterial Effects of Barberry Extract, Cinnamaldehyde and Nisin. Arch Hyg Sci 2019; 8 (2) :128-135
URL: http://jhygiene.muq.ac.ir/article-1-308-en.html

Combined Antilisterial Effects of Barberry Extract, Cinnamaldehyde and Nisin

Soghra Valizadeh ^*

¹, Javad Aliakbarlu¹

1- Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abstract: (2648 Views)

Background & Aims of the Study: Foodborne illnesses, resulting from consumption of contaminated foods, represent a substantial public health threat. Listeria monocytogenes is a foodborne pathogen bacteria and can cause serious problems in human. Because of this reason, Nowadays various compounds are being used to control these bacteria in foods. The aim of this study was to evaluate antilistarial potential of barberry extract, cinnamaldehyde (CA) and nisin, also to discover synergistic effects of dual combination of these compounds againt Listeria monocytogenes in culture medium.
Material and methods: The antilisterial activity of barberry extract, cinnamaldehyde (CA) and nisin was evaluated using agar well diffusion method and minimal inhibitory concentration (MIC), also Combined antilisterial activity was examined by fractional inhibitory concentration (FIC) and time-kill assays.
Results: In agar well diffusion method, nisin (400 IU/ml) and CA (40 µl/ml) produced the largest inhibition zones (20.75±0.25 and 32.0±1, respectively). MIC values of nisin, CA and barberry extract were 25 IU/ml, 0.312 µl/ml and 37.5 mg/ml, respectively. In time kill assay, the combination of nisin with CA in MIC concentration was found the best combination against L. monocytogenes and a 6 log reduction in bacterial count was obtained.

Conclusion: The results of this study revealed that nisin and CA have convenient antilisterial activity and combination of these two compounds in MIC concentration showed synergistic effects against Listeria monocytogenes.

Keywords: Listeria monocytogenes, Barberry Extract, Cinnamaldehyde, nisin

Full-Text [PDF 239 kb] (820 Downloads) | | Full-Text (HTML) (755 Views)

Type of Study: Original Article | Subject: Microbiology
Received: 2018/01/13 | Accepted: 2019/06/17 | Published: 2019/08/15

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