[Home ] [Archive]    
:: Volume 8, Issue 2 (Spring 2019 2019) ::
Arch Hyg Sci 2019, 8(2): 128-135 Back to browse issues page
Combined Antilisterial Effects of Barberry Extract, Cinnamaldehyde and Nisin
Soghra Valizadeh * , Javad Aliakbarlu
Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Abstract:   (1203 Views)
Background & Aims of the Study: Foodborne illnesses, resulting from consumption of contaminated foods, represent a substantial public health threat. Listeria monocytogenes is a foodborne pathogen bacteria and can cause serious problems in human. Because of this reason, Nowadays various compounds are being used to control these bacteria in foods. The aim of this study was to evaluate antilistarial potential of barberry extract, cinnamaldehyde (CA) and nisin, also to discover synergistic effects of dual combination of these compounds againt Listeria monocytogenes in culture medium.
 Material and methods: The antilisterial activity of barberry extract, cinnamaldehyde (CA) and nisin was evaluated using agar well diffusion method and minimal inhibitory concentration (MIC), also Combined antilisterial activity was examined by fractional inhibitory concentration (FIC) and time-kill assays.
Results: In agar well diffusion method, nisin (400 IU/ml) and CA (40 µl/ml) produced the largest inhibition zones (20.75±0.25 and 32.0±1, respectively). MIC values of nisin, CA and barberry extract were 25 IU/ml, 0.312 µl/ml and 37.5 mg/ml, respectively. In time kill assay, the combination of nisin with CA in MIC concentration was found the best combination against L. monocytogenes and a 6 log reduction in bacterial count was obtained.
Conclusion: The results of this study revealed that nisin and CA have convenient antilisterial activity and combination of these two compounds in MIC concentration showed synergistic effects against Listeria monocytogenes.
Keywords: Listeria monocytogenes, Barberry Extract, Cinnamaldehyde, nisin
Full-Text [PDF 239 kb]   (283 Downloads) |   |   Full-Text (HTML)  (241 Views)  
Type of Study: Original Article | Subject: Microbiology
Received: 2018/01/13 | Accepted: 2019/06/17 | Published: 2019/08/15
References
1. 1. Nyachuba DG. Foodborne illness: is it on the rise? Nutr Rev 2010;8(5):257-69. PubMed [DOI:10.1111/j.1753-4887.2010.00286.x]
2. Eliopoulos G. Synergism and antagonism. Infec Dis Clin North Am 1989; 3(3):399-406. PubMed
3. McLauchlin J. The relationship between Listeria and listeriosis. Food Control 1996; 7(4-5):187-93. Link [DOI:10.1016/S0956-7135(96)00038-2]
4. White DG, Zhao S, Simjee S, Wagner DD, McDermott PF. Antimicrobial resistance of foodborne pathogens. Microbes Infect 2002; 4(4): 405-12. PubMed [DOI:10.1016/S1286-4579(02)01554-X]
5. Gandhi M, Chikindas ML. Listeria: a foodborne pathogen that knows how to survive. Int J Food Microbiol 2007;113(1):1-15. PubMed [DOI:10.1016/j.ijfoodmicro.2006.07.008]
6. Liu D. Handbook of Listeria monocytogenes. New York: CRC Press; 2008. [DOI:10.1201/9781420051414]
7. Feniman C, Rall V, Doyama J, Júnior AF. Cell enumeration and visualisation by transmission electron microscopy of Lactobacillus rhamnosus treated with cinnamon (Cinnamomum zeylanicum B.) essential oil. Nat Prod Res 2012;26(18):1721-3. PubMed [DOI:10.1080/14786419.2011.605365]
8. Alves FC, Barbosa LN, Andrade BF, Albano M, Furtado FB, Pereira AFM, et al. Short communication: Inhibitory activities of the lantibiotic nisin combined with phenolic compounds against Staphylococcus aureus and Listeria monocytogenes in cow milk. J Dairy Sci 2016;99(3):1831-6. PubMed [DOI:10.3168/jds.2015-10025]
9. Shi C, Zhang X, Zhao X, Meng R, Liu Z, Chen X, et al. Synergistic interactions of nisin in combination with cinnamaldehyde against Staphylococcus aureus in pasteurized milk. Food Control 2017;71:10-6. Link [DOI:10.1016/j.foodcont.2016.06.020]
10. Pei RS, Zhou F, Ji BP, Xu J. Evaluation of combined antibacterial effects of eugenol, cinnamaldehyde, thymol, and carvacrol against E. coli with an improved method. J Food Sci 2009;74(7):M379-83. PubMed [DOI:10.1111/j.1750-3841.2009.01287.x]
11. Sanla Ead N, Jangchud A, Chonhenchob V, Suppakul P. Antimicrobial Activity of cinnamaldehyde and eugenol and their activity after incorporation into cellulose based packaging films. Packag Technol Sci 2012; 25(1): 7-17. [DOI:10.1002/pts.952]
12. Burt S. Essential oils: their antibacterial properties and potential applications in foods- a review. Int J Food Microbiol 2004;94(3):223-53. Link [DOI:10.1016/j.ijfoodmicro.2004.03.022]
13. Mills S, Stanton C, Hill C, Ross RP. New developments and applications of bacteriocins and peptides in foods. Annu Rev Food Sci Technol 2011;2:299-329. PubMed [DOI:10.1146/annurev-food-022510-133721]
14. Arques JL, Rodriguez E, Nunez M, Medina M. Antimicrobial activity of nisin, reuterin, and the lactoperoxidase system on Listeria monocytogenes and Staphylococcus aureus in cuajada, a semisolid dairy product manufactured in Spain. J Dairy Sci 2008;91(1):70-5. PubMed [DOI:10.3168/jds.2007-0133]
15. Caballero B, Trugo L, Finglas P. Nisin, Encyclopedia of food sciences and nutrition. Amsterdam, Netherlands: Elsevier Science BV; 2003.
16. Cotter PD, Hill C, Ross RP. Bacteriocins: developing innate immunity for food. Nat Rev Microbiol 2005;3(10):777-88. PubMed [DOI:10.1038/nrmicro1273]
17. Deegan LH, Cotter PD, Hill C, Ross P. Bacteriocins: biological tools for bio-preservation and shelf-life extension. Int Dairy J 2006;16(9):1058-71. Link [DOI:10.1016/j.idairyj.2005.10.026]
18. Shan B, Cai YZ, Brooks JD, Corke H. Antibacterial and antioxidant effects of five spice and herb extracts as natural preservatives of raw pork. J Sci Food Agric 2009;89(11):1879-85. Link [DOI:10.1002/jsfa.3667]
19. Fallahi J, Rezvni Moghaddam P, Nasiri Mohallati M. The effect of harvesting date on quantitative and qualitative of barberry (Berberis vulgaris). Iran Field Crops Res 2010;8:225-34. Link
20. Motalleb G, Hanachi P, Kua SH, Fauziah O, Asmah R. Evaluation of Phenolic contentand total antioxidant activity in Berberis vulgaris fruit extracts. J Biol Sci 2005;5(5):643-47. Link
21. Aliakbarlu J, Mohammadi S, Khalili S. A Study on antioxidant potency and antibacterial activity of water extracts of some spices widely consumed in Iranian diet. J Food Biochem 2014;38(2):159-66. Link [DOI:10.1111/jfbc.12034]
22. Barry AL, Craig WA, Nadler H, Reller LB, Sanders CC, Swenson JM. Methods for determining bacterial activity of antimicrobial agents, Approved guidline. The Clinical and Laboratory Standards Institute; 1999. Link
23. Lu F, Liu D, Ye X, Wei Y, Liu F. Alginate-calcium coating incorporating nisin and EDTA maintains the quality of fresh northern snakehead (Channa argus) fillets stored at 4 °C. J Sci Food Agric 2009;89(5):848-54. Link [DOI:10.1002/jsfa.3523]
24. Franklin R, Matthew A, Karen B. Performance standards for antimicrobial susceptibility testing: informational supplement (M100-S22). 22nd ed. Wayne , USA: CLSI; 2012. Link
25. Verma P. Methods for Determining Bactericidal Activity and Antimicrobial Interactions. Antimicrobial Susceptibility Testing Protocols. New York, USA: CRC Press; 2007. [DOI:10.1201/9781420014495.ch12]
26. Dos Santos AO, Ueda-Nakamura T, Dias BP, Veiga Junior VF, Pinto AC, Celso Vataru, et al. Antimicrobial activity of Brazilian copaiba oils obtained from different species of the Copaifera genus. Mem Inst Oswaldo Cruz 2008;103(3):277- 81. PubMed [DOI:10.1590/S0074-02762008005000015]
27. Ettayebi K, El Yamani J, Rossi-Hassani BD. Synergistic effects of nisin and thymol on antimicrobial activities in Listeria monocytogenes and Bacillus subtilis. FEMS Microbiol Lett 2000;183(1):191-5. PubMed [DOI:10.1111/j.1574-6968.2000.tb08956.x]
28. Campion A, Casey PG, Field D, Cotter PD, Hill C, Ross RP. In vivo activity of nisin A and nisin V against Listeria monocytogenes in mice. BMC Microbiol 2013;13(1):23. Link [DOI:10.1186/1471-2180-13-23]
29. Ukuku DO, Shelef LA. Sensitivity of six strains of Listeria monocytogenes to nisin. J Food Prot 1997;60(7):867-9. Link [DOI:10.4315/0362-028X-60.7.867]
30. Rasch M, Knöchel S. Variations in tolerance of Listeria monocytogenes to nisin, pediocin PA 1 and bavaricin A. Lett Appl Microbiol 1998;27(5):275-8. PubMed [DOI:10.1046/j.1472-765X.1998.t01-6-00449.x]
31. Harris LJ, Fleming HP, Klaenhammer TR. Sensitivity and resistance of Listeria monocytogenes ATCC 19115, Scott A, and UAL500 to nisin. J Food Prot 1991;54(11):836-40. PubMed [DOI:10.4315/0362-028X-54.11.836]
32. Zhou H, Fang J, Tian Y, Xiang Yang L. Mechanisms of nisin resistance in Gram-positive bacteria. Ann Microbiol 2014;64(2):413-20. Link [DOI:10.1007/s13213-013-0679-9]
33. Shen SX, Zhang TH, Yuan Y, Lin S, Xu J, Ye H. Effects of cinnamaldehyde on Escherichia coli and Staphylococcus aureus membran. Food Control 2015;47:196-202. Link [DOI:10.1016/j.foodcont.2014.07.003]
34. Feron V, Til H, De Vrijer F, Woutersen RA, Cassee FR,Van Bladeren PJ. Aldehydes: occurrence, carcinogenic potential, mechanism of action and risk assessment. Mutat Res/Gen Toxicol 1991;259(3-4):363-85. PubMed [DOI:10.1016/0165-1218(91)90128-9]
35. He J, Starr CG, Wimley WC. A Lack of Synergy Between Membrane-permeabilizing Cationic Antimicrobial Peptides and Conventional Antibiotics. Biochim Biophys Acta 2015;1848(1 0 0):8-15. PubMed [DOI:10.1016/j.bbamem.2014.09.010]
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA



XML     Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Valizadeh S, Aliakbarlu J. Combined Antilisterial Effects of Barberry Extract, Cinnamaldehyde and Nisin. Arch Hyg Sci. 2019; 8 (2) :128-135
URL: http://jhygiene.muq.ac.ir/article-1-308-en.html


Volume 8, Issue 2 (Spring 2019 2019) Back to browse issues page
Archives of Hygiene Sciences Archives of Hygiene Sciences
Persian site map - English site map - Created in 0.06 seconds with 29 queries by YEKTAWEB 4256