Volume 10, Issue 2 (Spring 2021)
Arch Hyg Sci 2021, 10(2): 143-154 |
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Rasouli A, Aghaei S S, Zargar M. Single-cell Oil Production Using Low-Cost Carbon Sources by Newly Isolated Kocuria Y205. Arch Hyg Sci 2021; 10 (2) :143-154
URL: http://jhygiene.muq.ac.ir/article-1-490-en.html
URL: http://jhygiene.muq.ac.ir/article-1-490-en.html
1- Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Qom Branch, Qom, Iran.
Abstract: (1189 Views)
Background & Aims of the Study: This study aimed to investigate, for the first time, the ability
of single-cell oil production from low-cost carbon sources using Kocuria Y205 native Iranian
bacterial isolates from the soil.
Materials and Methods: Whey and lignocellulose compounds were used as carbon sources
and yeast extract as a nitrogen source. Also, the isolated cultivation was done on Mineral Salts
Medium (MSM) culture media. Molecular analysis based on 16s rRNA gene sequence was
performed to identify isolated. Fourier-Transform Infrared Spectroscopy (FTIR) analysis was
used to confirm the presence of carbon groups. GC analysis was also used to identify the fatty
acids Sudan black. Finally, a Transmission Electron Microscopy (TEM) electron microscopy
image was taken to view the stored lipid granule.
Results: The highest rate of lipid production among all carbon sources in different periods of
cultivation by whey was 24.57% after 48 hours, and also, the highest rate of lipid production for
lignocellulose compounds was 15.29% after 48 hours.
Conclusion: This study shows that the newly isolated Kocuria Y 205 has excellent ability to
use whey and lignocellulose compounds as waste containing carbon resources for the growth,
production, and storage of microbial oil. It can be used in industrial applications, too.
of single-cell oil production from low-cost carbon sources using Kocuria Y205 native Iranian
bacterial isolates from the soil.
Materials and Methods: Whey and lignocellulose compounds were used as carbon sources
and yeast extract as a nitrogen source. Also, the isolated cultivation was done on Mineral Salts
Medium (MSM) culture media. Molecular analysis based on 16s rRNA gene sequence was
performed to identify isolated. Fourier-Transform Infrared Spectroscopy (FTIR) analysis was
used to confirm the presence of carbon groups. GC analysis was also used to identify the fatty
acids Sudan black. Finally, a Transmission Electron Microscopy (TEM) electron microscopy
image was taken to view the stored lipid granule.
Results: The highest rate of lipid production among all carbon sources in different periods of
cultivation by whey was 24.57% after 48 hours, and also, the highest rate of lipid production for
lignocellulose compounds was 15.29% after 48 hours.
Conclusion: This study shows that the newly isolated Kocuria Y 205 has excellent ability to
use whey and lignocellulose compounds as waste containing carbon resources for the growth,
production, and storage of microbial oil. It can be used in industrial applications, too.
Type of Study: Original Article |
Subject:
Microbiology
Received: 2020/12/14 | Accepted: 2021/02/16 | Published: 2021/02/9
Received: 2020/12/14 | Accepted: 2021/02/16 | Published: 2021/02/9
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Published by Qom University of Medical Sciences Press
eISSN: 2322-4916
pISSN: 2251-9203
Email: arch.hygiene[at]muq.ac.ir
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