Volume 10, Issue 1 (Winter 2021)                   Arch Hyg Sci 2021, 10(1): 67-74 | Back to browse issues page


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Fazli Qomi S M, Danaeefard M R, Farhang A B, Hosseini S P, Arast Y. Effect of Temperature on the Breeding Black Soldier Fly Larvae in Vitro for Basic Health-oriented Research. Arch Hyg Sci 2021; 10 (1) :67-74
URL: http://jhygiene.muq.ac.ir/article-1-470-en.html
1- MD in Veterinary Medicine, Faculty of Veterinary Medicine, Islamic Azad University of Garmsar Branch, Garmsar, Semnan, Iran
2- PhD in Toxicology, Faculty of Health, Qom University of Medical Sciences, Qom, Iran
Abstract:   (2064 Views)
Background & Aims of the Study: The prevalence of food insecurity in many countries and the challenges emerging to feed more than 9 billion people by 2050 have led the researchers to look for alternative sources of protein in human and animal diets. In this regard, today, the use of insects has attracted a lot of attention since they contain high nutritional value and help to preserve environmental resources. Among the various species of insects, particular attention has been paid to the black soldier fly (BSF) since it can consume from a variety of substrates, including organic waste. Various factors, such as temperature, humidity, density, light, and diet, are involved in the breeding of this insect. It seems that temperature is more effective in the breeding stages of this species than the other factors. Due to the insufficient information on finding the optimal temperature in breeding this species, this study was conducted to determine the mentioned factor in the maximum production and reproduction of black soldier flies to eliminate organic waste and turn it into valuable material in animal food.
Materials and Methods: Organic waste, including kitchen fruit and food, was used to feed the larvae. Adult flies were then reared in cotton net cages (40´40´40 cm) and under the temperature range of 25-35°C. Afterward, the eggs were collected by fine needles and transferred to a temperature-controlled incubator during the hatching stage to undergo experiments in the specified temperature range (i.e., 25-35°C). The larvae fed freely from the formulated diet (i.e., chicken feed) until the pre-pupal stage. The produced pupae were monitored for growth and survival in the temperature range of 25-35°C. The emergence of adult BSFs at different temperatures was examined after the completion of the pupal stage under controlled temperature. In this descriptive study after the completion of each insect's development stage, the percentage of insect survival in each stage of measured temperature condition was determined by estimating the proportion of the attribute present in the population.
Results: According to the results of this study, the highest hatching percentage (80%) was recorded at 30°C for 4 days, while the slowest growth period was obtained at 30°C for 13 days with a survival rate of 92%. It was also revealed that the highest pre-pupal and pupal survival rates were 82% at 30°C for 10 days and 77% at 30°C  for 7 days, respectively. The lifespan of adult flies at 30°C was reported to be 9 days. The statistical population of this consisted of 300 pupae at each temperature. The survival percentage was reported after the survived pupae were counted.
Conclusion: The results of this study showed that the growth and reproduction of BSFs were significantly affected by temperature. In this study, the optimum temperature in the breeding of BSFs was obtained as 30°C. Temperature can also affect the insect's biological life cycle, such as immaturity survival and adult lifespan, growth, fertility, gender ratio, and population growth parameters.
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Type of Study: Original Article | Subject: Environmental Health
Received: 2020/09/20 | Accepted: 2020/11/17 | Published: 2021/01/19

References
1. 1. Makkar, H.P., et al., State-of-the-art on use of insects as animal feed. Animal Feed Science and Technology, 2014. 197: p. 1-33. [DOI:10.1016/j.anifeedsci.2014.07.008]
2. Van Huis, A., Potential of insects as food and feed in assuring food security. Annual review of entomology, 2013. 58: p. 563-583. [DOI:10.1146/annurev-ento-120811-153704]
3. Alexandratos, N. and J. Bruinsma, World agriculture towards 2030/2050: the 2012 revision. 2012.
4. Rosegrant, M.W., S. Tokgoz, and P. Bhandary, The new normal? A tighter global agricultural supply and demand relation and its implications for food security. American Journal of Agricultural Economics, 2013. 95(2): p. 303-309. [DOI:10.1093/ajae/aas041]
5. Bruinsma, J., World Agriculture: towards 2015/2030: Summary Report. 2002: Food and Agriculture Organization of the United Nations (FAO).
6. Gustavsson, J., et al., Global food losses and food waste. 2011, FAO Rome.
7. Oonincx, D.G., et al., An exploration on greenhouse gas and ammonia production by insect species suitable for animal or human consumption. PloS one, 2010. 5(12): p. e14445. [DOI:10.1371/journal.pone.0014445]
8. Llagostera, P.F., et al., The use of insect meal as a sustainable feeding alternative in aquaculture: Current situation, Spanish consumers' perceptions and willingness to pay. Journal of Cleaner Production, 2019. 229: p. 10-21. [DOI:10.1016/j.jclepro.2019.05.012]
9. Van Huis, A., et al., Edible insects: future prospects for food and feed security. 2013: Food and Agriculture Organization of the United Nations.
10. Hoc, B., et al., Optimization of black soldier fly (Hermetia illucens) artificial reproduction. PloS one, 2019. 14(4): p. e0216160. [DOI:10.1371/journal.pone.0216160]
11. Burtle, G., et al., Mass Production of Black Soldier Fly Prepupae for Aquaculture Diets. A Manuscript for Aquaculture International. University of Georgia, Tifton Campus, Tifton, GA, 2012.
12. Lalander, C., et al., Effects of feedstock on larval development and process efficiency in waste treatment with black soldier fly (Hermetia illucens). Journal of cleaner production, 2019. 208: p. 211-219. [DOI:10.1016/j.jclepro.2018.10.017]
13. Koutsos, L., A. McComb, and M. Finke, Insect Composition and Uses in Animal Feeding Applications: A Brief Review. Annals of the Entomological Society of America, 2019. 112(6): p. 544-551. [DOI:10.1093/aesa/saz033]
14. Do, S., et al., 240 True nutrient and amino acid digestibility of black soldier fly larvae differing in life stage using the precision-fed cecectomized rooster assay. Journal of Animal Science, 2019. 97(Supplement_3): p. 64-65. [DOI:10.1093/jas/skz258.133]
15. Onsongo, V., et al., Insects for income generation through animal feed: Effect of dietary replacement of soybean and fish meal with black soldier fly meal on broiler growth and economic performance. Journal of economic entomology, 2018. 111(4): p. 1966-1973. [DOI:10.1093/jee/toy118]
16. Belghit, I., et al., Black soldier fly larvae meal can replace fish meal in diets of sea-water phase Atlantic salmon (Salmo salar). Aquaculture, 2019. 503: p. 609-619. [DOI:10.1016/j.aquaculture.2018.12.032]
17. Marshall, S., N. Woodley, and M. Hauser, The historical spread of the Black Soldier Fly, Hermetia illucens (L.)(Diptera, Stratiomyidae, Hermetiinae), and its establishment in Canada. The Journal of the Entomological Society of Ontario, 2015. 146.
18. Diener, S., C. Zurbrügg, and K. Tockner, Conversion of organic material by black soldier fly larvae: establishing optimal feeding rates. Waste Management & Research, 2009. 27(6): p. 603-610. [DOI:10.1177/0734242X09103838]
19. Bradley, S.W. and D. Sheppard, House fly oviposition inhibition by larvae ofHermetia illucens, the black soldier fly. Journal of Chemical Ecology, 1984. 10(6): p. 853-859. [DOI:10.1007/BF00987968]
20. Oliveira, F.R., K. Doelle, and R. Smith, External morphology of Hermetia illucens Stratiomyidae: Diptera (L. 1758) based on electron microscopy. Annual Research & Review in Biology, 2016: p. 1-10. [DOI:10.9734/ARRB/2016/22973]
21. Cranshaw, W. and D. Shetlar, Garden insects of North America: The ultimate guide to backyard bugs. 2017: Princeton University Press. [DOI:10.2307/j.ctt1qft28g]
22. Kim, W., et al., Biochemical characterization of digestive enzymes in the black soldier fly, Hermetia illucens (Diptera: Stratiomyidae). Journal of Asia-Pacific Entomology, 2011. 14(1): p. 11-14. [DOI:10.1016/j.aspen.2010.11.003]
23. De Smet, J., et al., Microbial community dynamics during rearing of black soldier fly larvae (Hermetia illucens) and impact on exploitation potential. Appl. Environ. Microbiol., 2018. 84(9): p. e02722-17. [DOI:10.1128/AEM.02722-17]
24. Nguyen, T.T., J.K. Tomberlin, and S. Vanlaerhoven, Ability of black soldier fly (Diptera: Stratiomyidae) larvae to recycle food waste. Environmental entomology, 2015. 44(2): p. 406-410. [DOI:10.1093/ee/nvv002]
25. Bondari, K. and D. Sheppard, Soldier fly, Hermetia illucens L., larvae as feed for channel catfish, Ictalurus punctatus (Rafinesque), and blue tilapia, Oreochromis aureus (Steindachner). Aquaculture Research, 1987. 18(3): p. 209-220. [DOI:10.1111/j.1365-2109.1987.tb00141.x]
26. Akhtar, Y. and M. Isman, Insects as an alternative protein source, in Proteins in food processing. 2018, Elsevier. p. 263-288. [DOI:10.1016/B978-0-08-100722-8.00011-5]
27. Spranghers, T., et al., Nutritional composition of black soldier fly (Hermetia illucens) prepupae reared on different organic waste substrates. Journal of the Science of Food and Agriculture, 2017. 97(8): p. 2594-2600. [DOI:10.1002/jsfa.8081]
28. Palma, L., et al., Managing high fiber food waste for the cultivation of black soldier fly larvae. npj Science of Food, 2019. 3(1): p. 1-7. [DOI:10.1038/s41538-019-0047-7]
29. Cai, M., et al., Bioconversion-Composting of Golden Needle Mushroom (Flammulina velutipes) Root Waste by Black Soldier Fly (Hermetia illucens, Diptera: Stratiomyidae) Larvae, to Obtain Added-Value Biomass and Fertilizer. Waste and biomass valorization, 2019. 10(2): p. 265-273. [DOI:10.1007/s12649-017-0063-2]
30. Kawasaki, K., et al., Evaluation of Black Soldier Fly (Hermetia illucens) Larvae and Pre-Pupae Raised on Household Organic Waste, as Potential Ingredients for Poultry Feed. Animals, 2019. 9(3): p. 98. [DOI:10.3390/ani9030098]
31. Mazza, L., et al., Management of chicken manure using black soldier fly (Diptera: Stratiomyidae) larvae assisted by companion bacteria. Waste Management, 2020. 102: p. 312-318. [DOI:10.1016/j.wasman.2019.10.055]
32. Myers, H.M., et al., Development of black soldier fly (Diptera: Stratiomyidae) larvae fed dairy manure. Environmental entomology, 2014. 37(1): p. 11-15. [DOI:10.1093/ee/37.1.11]
33. Liu, T., et al., Effects of black soldier fly larvae (Diptera: Stratiomyidae) on food waste and sewage sludge composting. Journal of Environmental Management, 2020. 256: p. 109967. [DOI:10.1016/j.jenvman.2019.109967]
34. Dzepe, D., et al., Influence of larval density, substrate moisture content and feedstock ratio on life history traits of black soldier fly larvae. Journal of Insects as Food and Feed, 2019: p. 1-8.
35. Sánchez-Muros, M.-J., F.G. Barroso, and F. Manzano-Agugliaro, Insect meal as renewable source of food for animal feeding: a review. Journal of Cleaner Production, 2014. 65: p. 16-27. [DOI:10.1016/j.jclepro.2013.11.068]
36. Bale, J.S., et al., Herbivory in global climate change research: direct effects of rising temperature on insect herbivores. Global change biology, 2002. 8(1): p. 1-16. [DOI:10.1046/j.1365-2486.2002.00451.x]
37. Saska, P., et al., Temperature effects on pitfall catches of epigeal arthropods: a model and method for bias correction. Journal of Applied Ecology, 2013. 50(1): p. 181-189. [DOI:10.1111/1365-2664.12023]
38. Goulson, D., et al., Predicting calyptrate fly populations from the weather, and probable consequences of climate change. Journal of Applied Ecology, 2005. 42(5): p. 795-804. [DOI:10.1111/j.1365-2664.2005.01078.x]
39. Salum, J., et al., Demographic parameters of the two main fruit fly (D iptera: Tephritidae) species attacking mango in C entral T anzania. Journal of Applied Entomology, 2014. 138(6): p. 441-448. [DOI:10.1111/jen.12044]
40. Logan, J., et al., An analytic model for description of temperature dependent rate phenomena in arthropods. Environmental Entomology, 1976. 5(6): p. 1133-1140. [DOI:10.1093/ee/5.6.1133]
41. Summers, C., R. Coviello, and A.P. Gutierrez, Influence of constant temperatures on the development and reproduction of Acyrthosiphon kondoi (Homoptera: Aphididae). Environmental Entomology, 1984. 13(1): p. 236-242. [DOI:10.1093/ee/13.1.236]
42. Gabre, R.M., F.K. Adham, and H. Chi, Life table of Chrysomya megacephala (Fabricius)(Diptera: Calliphoridae). Acta oecologica, 2005. 27(3): p. 179-183. [DOI:10.1016/j.actao.2004.12.002]
43. Karimi-Malati, A., et al., Life table parameters and survivorship of Spodoptera exigua (Lepidoptera: Noctuidae) at constant temperatures. Environmental Entomology, 2014. 43(3): p. 795-803. [DOI:10.1603/EN11272]

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