Antibacterial, Antioxidant, and Anticancer Activities of Biosynthesized Selenium Nanoparticles Using Two Indigenous Halophilic Bacteria

Tabibi, Maryam; Agaei, Seyed Soheil; Amoozegar, Mohammad Ali; Nazari, Razieh; Zolfaghari, Mohammad Reza

doi:10.52547/ArchHygSci.9.4.275

Volume 9, Issue 4 (Autumn 2020) Arch Hyg Sci 2020, 9(4): 275-286 | Back to browse issues page

‎ 10.52547/ArchHygSci.9.4.275

‎ 20.1001.1.22519203.2020.9.4.7.0

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Tabibi M, Agaei S S, Amoozegar M A, Nazari R, Zolfaghari M R. Antibacterial, Antioxidant, and Anticancer Activities of Biosynthesized Selenium Nanoparticles Using Two Indigenous Halophilic Bacteria. Arch Hyg Sci 2020; 9 (4) :275-286
URL: http://jhygiene.muq.ac.ir/article-1-463-en.html

Antibacterial, Antioxidant, and Anticancer Activities of Biosynthesized Selenium Nanoparticles Using Two Indigenous Halophilic Bacteria

Maryam Tabibi¹

, Seyed Soheil Agaei ^*

¹, Mohammad Ali Amoozegar²

, Razieh Nazari¹

, Mohammad Reza Zolfaghari¹

1- Department of Microbiology, Qom Branch, Islamic Azad University, Qom, Iran
2- Extremophiles Laboratory, Department of Microbiology, Faculty of Biology and Center of Excellence in Phylogeny of Living Organisms, College of Science, University of Tehran, Tehran, Iran

Abstract: (2452 Views)

Background & Aims of the Study: Selenium is an essential nutritional material used for the important functions of the human body. The issue of the production of selenium nanoparticles (SeNPs) was investigated in various fields, such as anticancer, antioxidant, and antibacterial activities.
Materials and Methods: In order to study antibacterial activity, the nutrient broth medium containing synthesized SeNPs in six different concentrations (i.e., 100, 50, 25, and 12.5 µM) was evaluated on six pathogenic bacteria. Then, the growth curves were drawn as an antibacterial assay in six pathogenic bacteria. In addition, the antioxidant effect was examined by the 2, 2-Diphenyl-1-picrylhydrazyl method. The stock solution of SeNPs was mixed with culture media in six different concentrations (i.e., 0.001, 0.01, 0.1, 1, 10, and 100 μM) and exposed on MCF-7 and HT-29 cell lines; therefore, the anticancer effects of SeNPs were assayed by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method.
Results: According to the obtained results, the most and least significant antibacterial effects of synthesized SeNPs were observed for Staphylococcus aureus (98%) and Klebsiella pneumonia (51.55%), respectively. The highest concentration of biosynthesized SeNPs can achieve a better outcome regarding the antioxidant activity. The growth of MCF-7 and HT-29 cancer cell lines were inhibited by synthesized SeNPs in a concentration of 100 µM.

Conclusion: Consequently, the results of the present study showed that SeNPs synthesized from indigenous halophilic bacteria could display anticancer, antioxidant, and antibacterial activities. This progress can assist in the treatment of different diseases.

Keywords: Antibacterial agents, Anticancer, Antioxidants, Halophile, Nanoparticles, Selenium

Full-Text [PDF 990 kb] (763 Downloads) | | Full-Text (HTML) (1087 Views)

Type of Study: Original Article | Subject: Microbiology
Received: 2020/08/2 | Accepted: 2020/09/7 | Published: 2020/10/1

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