Organophosphate Pesticide Exposure Reduced Serum Paraoxonase1 (PON1) Activity Which Correlated With Oxidative Stress in Pesticide Factory Workers

Alimohammadi , Mahshid; Soodi,  Maliheh; Gholami Fesharaki, Mohammad

doi:10.29252/ArchHygSci.8.2.88

Volume 8, Issue 2 (Spring 2019 2019) Arch Hyg Sci 2019, 8(2): 88-97 | Back to browse issues page

‎ 10.29252/ArchHygSci.8.2.88

‎ 20.1001.1.22519203.2019.8.2.7.9

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Alimohammadi M, Soodi M, Gholami Fesharaki M. Organophosphate Pesticide Exposure Reduced Serum Paraoxonase1 (PON1) Activity Which Correlated With Oxidative Stress in Pesticide Factory Workers. Arch Hyg Sci 2019; 8 (2) :88-97
URL: http://jhygiene.muq.ac.ir/article-1-364-en.html

Organophosphate Pesticide Exposure Reduced Serum Paraoxonase1 (PON1) Activity Which Correlated With Oxidative Stress in Pesticide Factory Workers

Mahshid Alimohammadi¹

, Maliheh Soodi ^*

¹, Mohammad Gholami Fesharaki²

1- Department of Toxicology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
2- DBepartment ofBiostatistics , Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran

Abstract: (3539 Views)

Background & Aims of the Study: Serum paraoxonase (PON1) is a potent antioxidant that is associated with the pathogenesis of several diseases. Also, it is reported that environmental factors can modulate the PON1 activity. In this study, the association between the Organophosphates (OP) exposure and plasma Paraoxonase/Arylesterase (PON1) activity and also OP-induced oxidative stress was investigated among pesticide manufacturing factory workers.
Materials and Methods: In this cross-sectional study, Plasma cholinesterase (PChE) and RBC Acetylcholinesterase activity (AChE) were measured as a biomarker of OP exposure. Also, the amount of malondialdehyde (MDA) was evaluated as a biomarker for lipid peroxidation and oxidative stress. The PON1activity was measured by two distinct substrates, Paraoxson and Phenylacetate, to measure paraoxonase (PONase) and arylesterase (AREase) activities of PON1 enzyme respectively.
Results: A significant decrease was observed inPChE and AChE activities in the blood sample of factory workers which confirmed OP exposure (p<0.001). Furthermore, the level of MDA increased that shown lipid peroxidation induced by OP (p<0.05). PONase and AREase activities decreased significantly in factory workers (p<0.05, p<0.001_). The reduction in AREase activity shown a correlation with the decrease in PChE and AChE activities (p<0.01), also a negative correlation was observed with AREase activity and lipid peroxidation (p<0.01). The Ponase activity only negatively correlated with lipid peroxidation (p<0.01).
Conclusion: Our study indicates that occupational OP pesticide exposure in factory workers causes a decrease in serum PON1 activity which is associated with oxidative stress. Decreased PON1 activity is involved in the pathogenesis of several diseases such as cardiovascular disease then it is recommended that PON1 activity of OP-pesticide workers has been periodically monitored.

Keywords: Occupational exposure, Organophosphate pesticide, Paraoxonase, Arylesterase, Oxidative stress

Full-Text [PDF 494 kb] (817 Downloads) | | Full-Text (HTML) (771 Views)

Type of Study: Original Article | Subject: Occuptional Health
Received: 2018/11/24 | Accepted: 2019/05/15 | Published: 2019/08/15

References

1. Suratman S, Edwards JW, Babina K. Organophosphate pesticides exposure among farmworkers: pathways and risk of adverse health effects. Rev Environ Health 2015;30(1):65-79. PubMed [DOI:10.2147/RMHP.S97733]

2. Mostafalou S, Abdollahi M. Pesticides and human chronic diseases: evidences, mechanisms, and perspectives. Toxicol Appl Pharmacol 2013;268(2):157-77. PubMed [DOI:10.1016/j.taap.2013.01.025]

3. McCauley LA, Anger WK, Keifer M, Langley R, Robson MG, Rohlman D. Studying health outcomes in farmworker populations exposed to pesticides. Environ Health Perspect 2006:114(6)953-60. PubMed [DOI:10.1289/ehp.8526]

4. Kamel F, Hoppin JA. Association of pesticide exposure with neurologic dysfunction and disease. Environ Health Perspect 2004:112(9):950-8. PubMed [DOI:10.1289/ehp.7135]

5. Alavanja MC, Hoppin JA, Kamel F. Health effects of chronic pesticide exposure: cancer and neurotoxicity. Annu Rev Public Health 2004;25:155-97. PubMed [DOI:10.1146/annurev.publhealth.25.101802.123020]

6. Voorhees JR, Rohlman DS, Lein PJ, Pieper AA. Neurotoxicity in preclinical models of occupational exposure to organophosphorus compounds. Front Neurosci 2017;10:590. PubMed [DOI:10.3389/fnins.2016.00590]

7. Mehrpour O, Karrari P, Zamani N, Tsatsakis AM, Abdollahi M. Occupational exposure to pesticides and consequences on male semen and fertility: A review. Toxicol Lett 2014;230(2):146-56. PubMed [DOI:10.1016/j.toxlet.2014.01.029]

8. Muñoz-Quezada MT, Lucero BA, Iglesias VP, Muñoz MP, Cornejo CA, Achu E, et al. Chronic exposure to organophosphate (OP) pesticides and neuropsychological functioning in farm workers: a review. International J Occup Environ Health 2016;22(1):68-79. PubMed [DOI:10.1080/10773525.2015.1123848]

9. Muñoz-Quezada MT, Lucero B, Iglesias V, Levy K, Muñoz MP, Achú E, et al. Exposure to organophosphate (OP) pesticides and health conditions in agricultural and non-agricultural workers from Maule, Chile. Int J Environ Health Res 2017;27(1):82-93. PubMed [DOI:10.1080/09603123.2016.1268679]

10. Mackness B, Durrington PN, Mackness MI. Human serum paraoxonase. Gen Pharmacol 1998;31(3):329-36. PubMed [DOI:10.1016/S0306-3623(98)00028-7]

11. Meek EC, Chambers HW, Pringle RB, Chambers JE. The effect of PON1 enhancers on reducing acetylcholinesterase inhibition following organophosphate anticholinesterase exposure in rats. Toxicology 2015;336:79-83. PubMed [DOI:10.1016/j.tox.2015.08.002]

12. Litvinov D, Mahini H, Garelnabi M. Antioxidant and anti-inflammatory role of paraoxonase 1: implication in arteriosclerosis diseases. N Am J Med Sci 2012;4(11):523-32. PubMed [DOI:10.4103/1947-2714.103310]

13. La Du BN. The human serum paraoxonase/arylesterase polymorphism. Am J Hum Genet 1988;43(3):227-9. PubMed

14. Afolabi OK, Wusu AD, Ogunrinola OO, Abam EO, Babayemi DO, Dosumu OA, et al. Paraoxonase 1 activity in subchronic low-level inorganic arsenic exposure through drinking water. Environ Toxicol 2016;31(2):154-62. PubMed [DOI:10.1002/tox.22030]

15. Devarajan A, Shih D, Reddy ST. Inflammation, infection, cancer and all that...the role of paraoxonases. Adv Exp Med Biol 2014;824:33-41. PubMed [DOI:10.1007/978-3-319-07320-0_5]

16. Camps J, García-Heredia A, Hernández-Aguilera A, Joven J. Paraoxonases, mitochondrial dysfunction and non-communicable diseases. Chem Biol Interact 2016;259(Pt B):382-7. PubMed [DOI:10.1016/j.cbi.2016.04.005]

17. Gawade G, Melinkeri R. Oxidative Stress and Paraoxonase (PON-1) Status in Diabetic Nephropathy. Int J Health Sci Res 2015;5(12):177-84. Link

18. Aviram M, Rosenblat M. Paraoxonases 1, 2, and 3, oxidative stress, and macrophage foam cell formation during atherosclerosis development. Free Radic Biol Med 2004;37(9):1304-16. PubMed [DOI:10.1016/j.freeradbiomed.2004.06.030]

19. Costa C, Gangemi S, Giambò F, Rapisarda V, Caccamo D, Fenga C. Oxidative stress biomarkers and paraoxonase 1 polymorphism frequency in farmers occupationally exposed to pesticides. Mol Med Rep 2015;12(4):6353-7. PubMed [DOI:10.3892/mmr.2015.4196]

20. Ranjbar A, Pasalar P, Abdollahi M. Induction of oxidative stress and acetylcholinesterase inhibition in organophosphorous pesticide manufacturing workers. Hum Exp Toxicol 2002;21(4):179-82. PubMed [DOI:10.1191/0960327102ht238oa]

21. Zepeda-Arce R, Rojas-Garcia AE, Benitez-Trinidad A, Herrera-Moreno JF, Medina-Diaz IM, Barron-Vivanco BS, et al. Oxidative stress and genetic damage among workers exposed primarily to organophosphate and pyrethroid pesticides. Environ Toxicol 2017;32(6):1754-64. PubMed [DOI:10.1002/tox.22398]

22. Costa LG, Cole TB, Vitalone A, Furlong CE. Measurement of paraoxonase (PON1) status as a potential biomarker of susceptibility to organophosphate toxicity. Clin Chim Acta 2005;352(1):37-47. PubMed [DOI:10.1016/j.cccn.2004.09.019]

23. Costa LG, Richter RJ, Li W-F, Cole T, Guizzetti M, Furlong CE. Paraoxonase (PON 1) as a biomarker of susceptibility for organophosphate toxicity. Biomarkers 2003;8(1):1-12. PubMed [DOI:10.1080/13547500210148315]

24. Ellman GL, Courtney KD, Andres V, Featherstone RM. A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol 1961;7(2):88-95. Link [DOI:10.1016/0006-2952(61)90145-9]

25. Ramirez-Santana M, Farias-Gomez C, Zuniga-Venegas L, Sandoval R, Roeleveld N, Van der Velden K, et al. Biomonitoring of blood cholinesterases and acylpeptide hydrolase activities in rural inhabitants exposed to pesticides in the Coquimbo Region of Chile. PloS one 2018;13(5):e0196084. Link [DOI:10.1371/journal.pone.0196084]

26. El-Demerdash FM, Nasr HM. Antioxidant effect of selenium on lipid peroxidation, hyperlipidemia and biochemical parameters in rats exposed to diazinon. J Trace Elem Med Biol 2014;28(1):89-93. PubMed [DOI:10.1016/j.jtemb.2013.10.001]

27. Ceron JJ, Tecles F, Tvarijonaviciute A. Serum paraoxonase 1 (PON1) measurement: an update. BMC Vet Res 2014;10(1):74. PubMed [DOI:10.1186/1746-6148-10-74]

28. Eckerson HW, Romson J, Wyte C, La Du B. The human serum paraoxonase polymorphism: identification of phenotypes by their response to salts. Am J Hum Genet 1983;35(2):214. PubMed

29. Eckerson HW, Wyte CM, La Du B. The human serum paraoxonase/arylesterase polymorphism. Am J Hum Genet 1983;35(6):1126. PubMed

30. Kapka-Skrzypczak L, Cyranka M, Skrzypczak M, Kruszewski M. Biomonitoring and biomarkers of organophosphate pesticides exposure - state of the art. Ann Agric Environ Med 2011;18(2):294-303. PubMed

31. Lionetto MG, Caricato R, Calisi A, Giordano ME, Schettino T. Acetylcholinesterase as a biomarker in environmental and occupational medicine: new insights and future perspectives Biomed Res Int 2013;2013 :321213. PubMed [DOI:10.1155/2013/321213]

32. He F. Biological monitoring of exposure to pesticides: current issues. Toxicol Lett 1999;108(2-3):277-83. PubMed [DOI:10.1016/S0378-4274(99)00099-5]

33. Dasgupta S, Meisner C, Wheeler D, Xuyen K, Thi Lam N. Pesticide poisoning of farm workers-implications of blood test results from Vietnam. Int J Hyg Environ Health 2007;210(2):121-32. PubMed [DOI:10.1016/j.ijheh.2006.08.006]

34. Surajudeen YA, Sheu RK, Ayokulehin KM, Olatunbosun AG. Oxidative stress indices in Nigerian pesticide applicators and farmers occupationally exposed to organophosphate pesticides. Int J Appl Basic Med Res 2014;4(Suppl 1): S37-40. PubMed [DOI:10.4103/2229-516X.140730]

35. Atherton KM, Williams FM, Egea Gonzalez FJ, Glass R, Rushton S, Blain PG, et al. DNA damage in horticultural farmers: a pilot study showing an association with organophosphate pesticide exposure. Biomarkers 2009;14(7):443-51. PubMed [DOI:10.3109/13547500903137265]

36. Aviram M, Billecke S, Sorenson R, Bisgaier C, Newton R, Rosenblat M, et al. Paraoxonase active site required for protection against LDL oxidation involves its free sulfhydryl group and is different from that required for its arylesterase/paraoxonase activities: selective action of human paraoxonase allozymes Q and R. Arterioscler Thromb Vasc Biol 1998;18(10):1617-24. PubMed [DOI:10.1161/01.ATV.18.10.1617]

37. Sorenson RC, Primo-Parmo SL, Kuo CL, Adkins S, Lockridge O, La Du BN. Reconsideration of the catalytic center and mechanism of mammalian paraoxonase/arylesterase. Proc Natl Acad Sci U S A 1995;92(16):7187-91. PubMed [DOI:10.1073/pnas.92.16.7187]

38. Sozmen EY, Sagin FG, Kayikcioglu M, Sozmen B. Oxidative stress & antioxidants and PON1 in health and disease. The Paraoxonases: Their Role in Disease Development and Xenobiotic Metabolism. Springer; 2008. p. 61-73. [DOI:10.1007/978-1-4020-6561-3_4]