en Environmental Health Environmental Health پژوهشي Original Article <strong>Background & Aims of the Study:</strong> Grape, a crucial agriculture crop of Malayer city, is affected by Vine cicada, Psalmocharias alhageos<span dir="RTL">. </span>&nbsp;Imidacloprid, a neonicotinoid insecticide, provides a good management of this insect. The aim of this research is residue persistence study of imidacloprid on grapes, to estimate its residue deposit, the half-life of degradation and safe pre-harvest consumption time.<br> <strong>Materials & Methods:</strong> Residues of imidacloprid were estimated in grape following two recommended types of spraying (80.0 g a.i. ha^-1) and duplicates the application rate (160.0 g a.i. ha^-1). Samples were collected at 1 h to 21 days after spraying of imidacloprid<span dir="RTL">.</span> The analyses were done by the QuECHERS technique, using HPLC-UV.<br> <strong>Results: </strong>The average initial concentration of imidacloprid on grapes found to be 10.58 and 17.56 mg kg^-1 at single and double dosages, respectively. These residues of imidacloprid decreased to 97.8% and 98.0%, respectively, at single and double dosages in 15 days, with a half-life period of 2.21 and 2.94 days. Residues of imidacloprid on grapes were less than its MRL value after 7 and 10 days of recommended and double dosage of spraying. Residues of imidacloprid in grape berries at harvest were discovered to below the determination limit.<br> <strong>Conclusions:</strong> Consequently, a waiting time of 7 and 10 days is usually recommended for safe consumption of grapes once imidacloprid spraying. Acceptable daily intake (ADI) of imidacloprid is 0.06 mg kg^-1 body weight day^-1. According to the results of this study, the employment of imidacloprid on the grape looks to be toxicologically acceptable.<br> &nbsp; Food Safety, Grape, Imidacloprid, Maximum Residue Limit (MRL), Confidor, Toxin, Safe (QuECHERS) Method, Iran 165 173 http://jhygiene.muq.ac.ir/browse.php?a_code=A-10-178-2&slc_lang=en&sid=1 Nasrin Hassanzadeh nasrinhassanzadeh@gmail.com 0000-0003-4530-2312 Yes Environmental Science Department Faculty of Natural Resources & Environment Malayer University Nader Bahramifar 0000-0002-2733-4057 No Environmental Science Department School of Natural Resources & Marine Sciences, Tarbiat Modares University Farzaneh Mohammad Zaheri 0000-0002-3735-8685 No Department of the Environment, College of Basic Science, Hamadan Branch, Islamic Azad University