en Environmental Health Environmental Health پژوهشي Original Article <p></p> <table align="left" cellpadding="0" cellspacing="0" hspace="0" vspace="0"> <tbody> <tr> <td align="left"><strong>Background & Aims of the Study:</strong> The objective of this study was to investigate the seed pre-treatment with salicylic acid (SA) on tolerance and remediation ability of Brassica oleracea var. acephala under lead and zinc stresses.<br> <strong>Materials and Methods:</strong> Present study was conducted to evaluate phyto-accumulative ability of Brassica oleracea var. acephala in heavy metal concentrations. After seed disinfection, the seeds were soaked in the solution of SA (0, 200 and 300 mgL^-1) for 6 hours and cultured in media with different concentrations ZnSO₄ and Pb(NO₃) (0, 50, 100 and 200 mgL^-1). Some germination indices such as the shoot to root ratio, leaf width, fresh and dry weight, chlorophyll content and absorption of heavy metal by seedling were investigated, after 14 days.<br> <strong>Results:</strong> The results showed that with increasing metal density, almost all of germination indices decreased significantly in comparison with the control. The most heavy metals accumulation was observed in seed pre-treatment with 300 mg L^-1 SA and 100 mg L^-1 metals treatment. By increasing the concentration of lead and zinc in the medium, the accumulation of metals was increased significantly in the plants. So, in the treatment of 100 mg L^-1 of each metal, the accumulation of lead and zinc were 8500.5 and 1085.1 mg kg^-1 in dry weight respectively.<br> <strong>Conclusions:</strong> The results show that that ornamental Kale can be used as a hyperaccumulator plant for lead and zinc in polluted regions in this study. The Ornamental kale would be a high biomass crop that can accumulate the contaminant of lead and zinc in the soil.</td> </tr> </tbody> </table> <p><div style="clear:both;"></div></p> Germination indices, Heavy metals, Ornamental Kale, Salicylic acid, Iran 1 11 http://jhygiene.muq.ac.ir/browse.php?a_code=A-10-120-1&slc_lang=en&sid=1 Mina Taghizadeh m-taghizadeh@araku.ac.ir Yes Department of Horticultural Science, Faculty of Agriculture and Natural Resources, Arak University, Arak, Iran. Mousa Solgi 0000-0003-0494-8782 No Department of Medical Biotechnology, National Institute of genetic engineering and biotechnology, Tehran, Iran Maryam Karimi No National Institute of genetic engineering and biotechnolog Mohammad Hosein Sanati 0000-0002-0292-7673 No National Institute of genetic engineering and biotechnolog Shohreh Khoshbin 0000-0002-3974-319X No Arak University