Volume 13, Issue 3 (Summer 2024)                   Arch Hyg Sci 2024, 13(3): 107-116 | Back to browse issues page


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Najafi M, Sari A A, Kalantari-Hesari A. Evaluation of Unauthorized Tissues in Minced Meat and Raw Koobideh Kebab in the West Provinces of Iran. Arch Hyg Sci 2024; 13 (3) :107-116
URL: http://jhygiene.muq.ac.ir/article-1-718-en.html
1- MSc graduate, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
2- Associate Professor, Department of Food Hygiene and Quality Control, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
3- Associate Professor, Department of Basic Sciences, Faculty of Veterinary Medicine, Bu-Ali Sina University, Hamedan, Iran
Abstract:   (186 Views)
Background & Aims: Nowadays, the consumption of meat products is on the rise. According to the national standard of Iran, these products must be free of unauthorized tissues such as the gastrointestinal tract of livestock and poultry, reproductive organs, liver, lungs, breasts, etc.
Materials and Methods: In the present study, conducted in the spring of 2024, 75 raw Koobideh Kebab and 60 minced meat samples were randomly collected from Hamedan, Sanandaj, and Kermanshah cities in Iran. Tissue cross-sections were prepared from all samples and stained using the hematoxylin-eosin staining method. The slides were examined under a light microscope, and Image Pro Plus (version 6.0) software was utilized to determine the quantities of skeletal muscles, fat, plant, smooth muscles, and connective tissues. Data were analyzed using SPSS (version 25.0) software with t-tests and one-way analysis of variance (One-Way ANOVA).
Results: The results indicated that 14 samples (56%) in Hamedan province, 16 samples (80%) in Kurdistan province, and 23 samples (76.66%) in Kermanshah province contained at least one unauthorized tissue, such as bone, skin, cartilage, gizzard, heart, viscera, and parasitic cysts.
Conclusion: Given the significant presence of unauthorized tissues in raw Koobideh Kebab and minced meats, it is imperative to enhance producers' awareness to improve product quality and establish a system of ongoing inspection and health control in production units.


 
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Type of Study: Original Article | Subject: General
Received: 2025/03/11 | Accepted: 2025/04/26 | Published: 2024/10/3

References
1. References 1. Rokni, N, Rezaian M, Noori N, Ebrahimpoor F. Detection of unauthorized tissue in some of cured meat products of Tehran with histological method. Pajouhesh Va Sazandegi, 2004;65: 2-5. Link
2. Mataragas M, Skandamis PN, Drosinos EH. Risk profiles of pork and poultry meat and risk ratings of various pathogen/product combinations. Int J Food Microbiol. 2008;126(1-2): 1-12. doi: 10.1016/j.ijfoodmicro.2008.05.014 [DOI:10.1016/j.ijfoodmicro.2008.05.014]
3. Asadi MR, Taghavi M, Hesari AK, Ghorbanzadeh B.The role of histological test in reducing the use of unauthorized tissues in meat products between years of 2014 and 2017. J Vet Res Biol Produc. 2020; 33(3): 31-40. doi:10.22092/vj.2019.116357.1387
4. Liscio C, Hopley CJ. Development of a reference measurement procedure and certified reference material for the determination of hydroxyproline in meat. Food Anal Methods. 2016;9: 1461-1469. doi:10.1007/s12161-015-0329-x [DOI:10.1007/s12161-015-0329-x]
5. Sadeghi E, Khazaei M, Almasi A, Shariatifar N, Bohlouli Oskoii S, Tahvilian R. Recognition of ilegal tssues in the meat Products from Kermanshah supply centers during the years 2009-2010. Intern Med Today. 2011;17(1): 55-59. Link
6. Veuthey T, Herrera G, Dodero VI. Dyes and stains: from molecular structure to histological application. Front Biosci (Landmark Ed). 2014;19(1):91-112. doi: 10.2741/4197 [DOI:10.2741/4197]
7. Daghighian R, Javadi A, Safavi S. Histological and chemical evaluation of frauds in ground meat used for kebab in Tabriz. J Food Hyg. 2016;6(1):15-26. Link
8. Izadi F, Sadeghinezhad J, Hajimohammadi B, Taghipour-zahir S, Fallahzadeh H, Sheibani M et al. Detection of unauthorized tissues in trade frozen minced meat marketed in Yazd with histological method. Tolooebehdasht. 2016; 14 (6) :423-431. Link
9. Abbasy-Fasarani M, Hosseini H, Jahed-Khaniki G, Adibmoradi M, Eskandari S. Histological study of industrial hamburgers containing 30 and 60 percent meat for presence of unpermitted edible tissues and correlation of this factor to meat connective tissue chemical indices. Iranian J Nutr Sci Food Technol. 2013; 7(5): 311-318. Link
10. Jahed Khaniki G, Rokni N. Histological study of unpermitted tissues in heated meat products by using of Masson's trichrome stain. Pajouhesh and Sazandegi. 2006; 19(4):96-102. Link
11. Latorre R, Sadeghinezhad J, Hajimohammadi B, Izadi F, Sheibani M. Application of morphological method for detection of unauthorized tissues in processed meat products. J Food Qual Hazards Control. 2015;2(2): 71-74. Link
12. Asadi MR, Kalantari-Hesari A, Ghaemmaghami SS, Mosleh N, Ghorbanzadeh B, Abdi P. Meat products components using histological method and image analysis software. Vet Res Biol Produc. 2023;36(1):102-112. doi: 10.22092/vj.2022.358361.1966
13. Kalantari-Hesari A, Babaei M, Nourian AR. Esfandiyari K, Elmi T, Soltani S, et al. Modified methods to simplification histochemical, immunohistochemical, and hematoxylin-eosin staining. Iran Vet J. 2022;18(3): 63-73. doi:10.22055/IVJ.2022.304383.2398
14. Mokhtar D, AbdEl-Aziz DM, Youssef H, Taha A. Applied histological and chemical analysis for detection of adulteration of minced meat and sausage. J Adv Microsc Res. 2018;13(3):345-53. doi:10.1166/jamr.2018.1401 [DOI:10.1166/jamr.2018.1401]
15. Prayson B, McMahon JT, Prayson RA. Fast food hamburgers: what are we really eating? Ann Diagn Pathol. 2008;12(6):406-409. doi: 10.1016/j.anndiagpath.2008.06.002 [DOI:10.1016/j.anndiagpath.2008.06.002]
16. Abdel Hafeez H, Zaki R, El-Mageed A. Applying light, histochemical and scanning histological methods for the detection of unauthorized animal and herbal content in street meat sandwich: What is in the sandwich we eat? J Food Process Technol. 2016; 7(12): 1-11. Link [DOI:10.4172/2157-7110.1000643]
17. Rokni, N., M. Rezaian, and A.J.J.o.V.M. Dayani Dardashti, Tehran University, Histological histometrical study of different heated sausages. J. Vet. Med., 1997. 52(1): p. 95-103 Link.
18. Ghazanfari M, Hagimohammadi B, Eskandari S, Karimiyan Khosroshahi N. Investigating the use of unauthorized tissues in handmade burgers in Tehran. Tolooebehdasht, 2018;17(1): 73-81. Link
19. Kavousi Nejad M, Fazlara A, Morowati H. Detection of unauthorized tissue in hamburger with histological method. Proceeding of the 15 th Congress of Veterinary of Iran. 2008. Link

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