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:: Volume 2, Number 1 (Winter 2013 2013) ::
Arch Hyg Sci 2013, 2(1): 1-6 Back to browse issues page
Characterization of the Extended-Spectrum beta-Lactamase Producers among Non-Fermenting Gram-Negative Bacteria Isolated from Burnt Patients
Mojdeh Hakemi Vala, Masoumeh Hallajzadeh, Fatemeh Fallah *, Ali Hashemi, Hossein Goudarzi
Pediatric Infections Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Abstract:   (441 Views)

Background & Aims of the Study: Extended-spectrum beta-Lactamases (ESBLs) represent a major group of beta-lactamases which are responsible for resistance to oxyimino-cephalosporins and aztreonam and currently being identified in large numbers throughout the world. The objective of this study was to characterize ESBL producers among non-fermenter gram-negative bacteria isolated from burnt patients.

Materials &Methods: During April to July 2012, 75 non-fermenter gram-negative bacilli were isolated from 240 bacterial cultures collected from wounds of burnt patients admitted to the Burn Unit at Shahid Motahari Hospital (Tehran, Iran). Bacterial isolation and identification was done using standard methods. Antimicrobial susceptibility testing was performed by disk diffusion method for all strains against selected antibiotics and minimum inhibitory concentration was determined by microdilution test. The ability to produce ESBL was detected through double disk synergy test among candidate strains.

Results: Of 75 non-fermenter isolates, 47 Pseudomonas aeruginosa and 28 Acinetobacter baumannii were identified. The resistance of P. aeruginosa isolates to tested antibiotics in antibiogram test were 100% to cefpodoxime, 82.98% to ceftriaxone, 78.73% to imipenem, 75% to meropenem, 72.72% to gentamicin, 69.23% to ciprofloxacin and aztreonam, 67.57% to cefepime, 65.95% to ceftazidime, and 61.53% to piperacillin. The results for Acinetobacter baumannii were 100% to ceftazidime, cefepime, ciprofloxacin, imipenem, meropenem, cefpodoxime, and cefotaxim, 96.85% to gentamicin, 89.65% to ceftriaxone, 65.51% to aztreonam, and 40% to piperacillin. Double disk synergy test showed that 21 (28%) of non-fermenter isolates were ESBL producer.

Conclusions: None of the third or fourth generation of cephalosporins is suitable for treatment of Pseudomonas aeruginosa and Acinetobacter baumannii isolated from burnt patients.

Keywords: Acinetobacter baumanni, beta-Lactamases, Drug Resistance, Iran, Patients, Burnt, Pseudomonas aeruginosa
Full-Text [PDF 268 kb]   (427 Downloads)    
Type of Study: Research | Subject: General
Received: 2016/02/13 | Accepted: 2016/02/23 | Published: 2016/02/23
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Hakemi Vala M, Hallajzadeh M, Fallah F, Hashemi A, Goudarzi H. Characterization of the Extended-Spectrum beta-Lactamase Producers among Non-Fermenting Gram-Negative Bacteria Isolated from Burnt Patients. Arch Hyg Sci. 2013; 2 (1) :1-6
URL: http://jhygiene.muq.ac.ir/article-1-7-en.html
Volume 2, Number 1 (Winter 2013 2013) Back to browse issues page
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